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Macronutrients in Crop Production

When growing crops of any type, it’s important to understand the required inputs in order to receive the desired yields. One of these inputs, arguably the most important and critical one, revolves around nutrient management. All plants have these requirements, whether it be crops grown for biofuels, fruit production, or landscape ornamentals. Each plant needs […]

via Macronutrients in Crop Production — Iowa Agriculture Literacy

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Single-Particle Inductively Coupled Plasma Mass Spectrometry for Characterization of Engineered Nanoparticles

With the rapid development of nanotechnology, consumer products containing metallic engineered nanoparticles (ENPs) are commonly available in the market. These particles subsequently are released into the environment, and uptake of these materials by human is very likely. Methods for detecting, quantifying, and characterizing these materials in complex matrices are critical for the eventual understanding of their implications to human health and environment. To assess their effect on the body, reliable and fast detection of these nanoparticles in very low amount becomes increasingly important. Single-particle ICP-MS has emerged (spICP-MS) as a useful tool for characterization of metal-containing nanoparticles. This technique is reliable and fast for counting and sizing particles at lowest concentrations while simultaneously distinguishing between dissolved and particulate analytes.

The chapter describing about this technique has been published in the book “Microbial Nanobionics” volume 2 Basic Research and Applications edited by  Dr. Ram Prasad.

https://link.springer.com/chapter/10.1007/978-3-030-16534-5_2

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Single-Particle Inductively Coupled Plasma Mass Spectrometry for Characterization of Engineered Nanoparticles

Single Cell-Inductively Coupled Plasma-Mass Spectrometry 

Single Cell-Inductively Coupled Plasma-Mass Spectrometry

(SC-ICP-MS)

An emerging technique for metal content analysis in single cells

SC-ICP-MS_Cancer

sc icpmspesc icpms350

Source: LCGC WEBCASTS

A New Era in Molecular Biology: “CRISPR/Cas9” and Targeted Gene Editing

A New Era in Molecular Biology: CRISPR/Cas9 and Targeted Genome Editing

GenomeEditing

The development of efficient and reliable ways to make precise, targeted changes to the genome of living cells is a long-standing goal for biomedical researchers. Recently, a new tool based on a bacterial CRISPR-associated protein-9 nuclease (Cas9) from Streptococcus pyogenes has generated considerable excitement. This follows several attempts over the years to manipulate gene function, including homologous recombination and RNA interference (RNAi). RNAi became a laboratory staple enabling inexpensive and high-throughput interrogation of gene function but it is hampered by providing only temporary inhibition of gene function and unpredictable off-target effects. Other recent approaches to targeted genome modification – zinc-finger nucleases (ZFNs), and transcription-activator like effector nucleases (TALENs) enable researchers to generate permanent mutations by introducing doublestranded breaks to activate repair pathways. These approaches are costly and time-consuming to engineer, limiting their widespread use, particularly for large scale, high-throughput studies

The functions of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and CRISPR-associated (Cas) genes are essential in adaptive immunity in select bacteria and archaea, enabling the organisms to respond to and eliminate invading genetic material. These repeats were initially discovered in the 1980s in E. coli, but their function wasn’t confirmed until 2007 by Barrangou and colleagues, who demonstrated that S. thermophilus can acquire resistance against a bacteriophage by integrating a genome fragment of an infectious virus into its CRISPR locus. Three types of CRISPR mechanisms have been identified, of which type II is the most studied. In this case, invading DNA from viruses or plasmids is cut into small fragments and incorporated into a CRISPR locus amidst a series of short repeats (around 20 bps). The loci are transcribed, and transcripts are then processed to generate small RNAs (crRNA – CRISPR RNA), which are used to guide effector endonucleases that target invading DNA based on sequence complementarity.

DNA Metabarcoding: A Rapid Method for Biodiversity Assessment

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DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample containing entire organisms. This offers unprecedented scientific and operation opportunities in order to understand biodiversity distribution and dynamics in a better way. Managing the health of global ecosystems requires detailed inventories of species and a good understanding of the patterns and trends of biodiversity. Evolutionary and ecological studies often rely on our ability to identify the species involved in the process under investigation or our capacity to provide robust biodiversity estimates. For about three centuries, the acquisition of biodiversity data was based on morphological characterization of plants and animals. The idea of identifying species on the basis of molecular markers emerged soon after the advent of molecular biology. Early methods involved the use of hybridization, restriction enzyme digestion or other molecular probes. DNA-based species identification was introduced by Arnot et al. and further development, standardized and advanced by Hebert et al. The ability to extract and store DNA for prolonged periods of time provides a unique opportunity to assess the evolution of biodiversity over time in relation to global change and to develop concrete measures to reserve these features. For more details, please click here.
DNA Metabarcoding

Nanotechnology: An Agricultural Paradigm

This book highlights the implications of nanotechnology and the effects of nanoparticles on agricultural systems, their interactions with plants as well as their potential applications as fertilizers and pesticides. It also discusses how innovative, eco-friendly approaches to improve food and agricultural systems lead to increased plant productivity. Further, it offers insights into the current trends and future prospects of nanotechnology along with the benefits and risks and their impact on agricultural ecosystems. contentNanomaterials in agriculture reduce the amount of chemical products sprayed by means of smart delivery of active ingredients; minimize nutrient losses in fertilization; and increase yields through optimized water and nutrient management. There is also huge potential for nanotechnology in the provision of state-of-the-art solutions for various challenges faced by agriculture and society, both today and in the future.

Chapter 12

Nanotechnology for Enhancing Crop Productivity

Suresh Kaushik and S.R. Djiwanti

Agriculture is currently facing a number of challenges like low nutrient use efficiency, stagnation in crop yields, multi-nutrient deficiencies, climate change, and water availability. One of the frontier technologies like nanotechnology can be explored to detect precisely and supply the accurate quantity of plant nutrients and pesticides to enhance crop productivity in agriculture. Nanotechnology involves the designing, production, characterization and application of devices, structures, and systems by controlling the size and shape at nanometer scale. Nanotechnology using nanodevices and nanomaterials provides new avenues for potential novel applications in agriculture such as efficient delivery of pesticide and fertilizer using nanomaterial-based formulations such as nano-fertilizers, nano-pesticides, and nano-herbicides. New innovative smart delivery systems and sensitive nano-biosensor-based technology have great potential to solve the problems faced in crop production. This chapter summarizes some new developments in smart delivery systems and nano biosensor-based technology for enhancing crop productivity.

Genetic Improvements of traits for enhancing NPK Acquisition and Utilization Efficiency in Plants

Book title: Plant Macronutrient Use Efficiency: Molecular and Genomic Perspectives in Crop Plants

Editors: Mohammad Anwar Hossain, Takehiro Kamiya, David J. Burrit, Lam-Son Phan Tran and Toru Fujiwara

Publisher: Academic Press, Elsevier

Mybook

Genetic Improvements of traits for enhancing NPK Acquisition and Utilization Efficiency in Plants

Inductively Coupled Plasma-Mass Spectrometry : A Rapid Technique for Multi-Elements Determination at the Ultra-Trace Level

 

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Inductively coupled plasma mass spectrometry (ICP-MS) is a type of mass spectrometry which is capable of detecting metals and several non-metals at concentrations as low as one part per trillion. ICP-MS is undoubtedly the fastest growing trace element technique available today. It allows determination of elements with atomic mass ranges 7 to 250. It is able to detect the elements upto part per trillion levels and this ability to carry out rapid multi-elements  determination at the ultra-trace level  have made it very popular in diverse range of applications areas  including environment, geochemical, semiconductor, metallurgical, nuclear, chemical, climatic and biotechnology. In recent years, industrial and biological monitoring has presented major need for metal analysis by ICP-MS. Other uses is in the medical and forensic field, specifically, toxicology and heavy metal poisoning.

For basics of ICP-MS working, please click on the following link…..

https://wordpress.com/page/gingerfingers.wordpress.com/438

 

Opening Black Box of Soil Microbial Diversity through Molecular Techniques

tree_of_life

Biodiversity is generally defined as the variety and variability of living organisms and the ecosystems in which this occurs. The variability of life in the soil encompasses not only plants and animals but also the invertebrates and microorganisms that are interdependent on one another and the higher plants they support. Biodiversity is composed of three interrelated elements: genetic, functional and taxonomic diversity as shown in Figure 1. Taxonomic diversity i.e. the number of species forms an important part of an ecosystem’s diversity and is controlled by the genetic diversity. Genetic diversity can be much more than the number of recognized species. Hence, several species may have the same functions, resulting in functional redundancy. Some species may also interact to perform functions not possible by any single species. Therefore, biodiversity is the interaction of all these elements.

Soil biodiversity is more extensive than any other environment on the globe when all living forms are considered. The soil biota contains representations of all groups of microorganisms, fungi, bacteria, algae and viruses, as well as the microfauna such as protozoa and nematodes. The total diversity is equal to greater than any coral reef or rain forest. Soil algae and protozoa, like higher plants and animals, can be identified by their morphology. Fungi and bacteria, however, require more extensive biochemical and genetic analysis to enable identification.

It has been estimated that only between 1 and 5% of all microorganisms on the earth have been named and classified. A large proportion of these unknown species is thought to reside in the soil. The possible numbers of existing species of different groups are 1.5 million species of fungi, 300,000 species of bacteria, 400,000 species of nematodes and 40,000 species of protozoa. New molecular techniques have been used to estimate that single gram of soil probably contains several thousand bacterial species.

 Opening Black Box of Soil Microbial Diversity through Molecular Techniques

 

 

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